Separation and Purification Techniques

MCAT Basics (from MedSchoolCoach)

MedSchoolCoach

Science

4.6 • 612 Ratings

🗓️ 23 January 2025

⏱️ 63 minutes

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Summary

This episode of MCAT Basics covers several separation and purification techniques you may see on the MCAT. We’ll cover these techniques in three distinct categories: small molecules, proteins, and cells.

Within the small molecule category, we’ll discuss extraction, crystallization and recrystallization, and chromatography (TLC and HPLC).

For proteins, we cover SDS-page, precipitation, soelectric focusing, and chromatography variations. Lastly, we’ll explain, within the world of cells, centrifugation, flow cytometry, and microfluidics.

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[00:00] MCAT Tutorig from MedSchoolCoach

[00:34] Welcome to MCAT Basics

[01:07] Topics covered in this episode

[02:24] Techniques for small molecules - extraction, crystallization and recrystallization, and chromatography (TLC and HPLC)

[28:24] Techniques for proteins - SDS-page, precipitation, isoelectric focusing, and chromatography variations

[54:47] Techniques for cells - centrifugation, flow cytometry, and microfluidics.

Transcript

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0:00.0	Welcome to MCAT basics, your ultimate guide to the essential topics you need to master for the MCAT,
0:07.3	brought to you by the physicians at med school coach.
0:10.2	Every week, Sam Smith breaks down high-yield MCAT topics, ensuring you're primed for success on test day.
0:16.7	Join Sam as we explore the most crucial subjects outlined by the AAMC,
0:21.7	pulled from official practice materials and third-party resources.
0:24.5	Get ready to elevate your MCAT game with topics tailored to maximize your score potential.
0:31.6	Hello, I'm Sam Smith.
0:34.1	This podcast is going to cover separation and purification techniques.
0:38.6	I'm going to focus on kind of three categories.
0:41.6	That is the separation and purification of organic molecules.
0:45.4	And then I'll talk about separation and purification of proteins.
0:49.3	And then lastly, I'll go into the separation and purification of cells.
0:54.7	And so you're mainly going to see this material come up in two out of the four MCAT sections.
0:59.8	Those sections would be the bio-biochem section and the phys-chem section.
1:05.0	Of course, you'll see probably in the phys-chem section more of the organic molecule separation, purification, and then probably in the biochem section you'll see more of the organic molecule separation purification and then probably in
1:12.8	the biochem section you'll see more of the protein stuff and I threw in this cell separation
1:17.0	and purification techniques because it's interesting and you know I haven't seen it too much on the
1:22.3	MCAP but I could definitely see them throwing it into the bio biochem section so just keep that in
1:27.1	mind all the stuff is pretty important it'll bring you back to O Chem lab see them throwing it into the bio-biotechem section. So just keep that in mind.
1:28.9	All this stuff is pretty important.
1:35.0	It'll bring you back to O-Cem lab, bio-chem, a lot of these different lab classes.
1:38.0	But definitely I've seen these come up on the MCAT, so it's important.
	...

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