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The Science of Everything Podcast

Episode 68: Protein Structure and Function

The Science of Everything Podcast

James Fodor

Social Sciences, Natural Sciences, Science

4.8819 Ratings

🗓️ 30 November 2014

⏱️ 54 minutes

🧾️ Download transcript

Summary

An overview of the structure and function of proteins. Beginning with a discussion of some key research methods for studying proteins, including column chromatography, electrophoresis, and x-ray crystallography, we then discuss the structure of proteins, with a focus on secondary structure, motifs, structural domains, and quaternary structure. The episode concludes with a look at protein function, including protein folding, denaturation, enzymatic function, and allosteric regulation. Recommended pre-requisites are Episode 18: Biochemistry Basics, and Episode 10: The Cell. Episode 32: Light and Optics may also be helpful for the crystallography portion.

Transcript

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0:00.0

Oh, wow, oh, oh, whoa, oh, wow.

0:13.0

Oh, wow.

0:15.0

Oh, wow. Hello, you're listening to The Sides of Everything podcast, episode 68, protein structure and function.

0:40.2

I'm your host, James Fodor.

0:42.0

Recommended pre-listening for this episode is episode 18, Biochemistry Basics.

0:47.3

Episode 10 on the cell may also be slightly useful.

0:50.7

So in this episode, we're going to look at proteins.

0:53.8

We talked about them briefly in episode 18 and maybe it touched on them otherwise episode, we're going to look at proteins. We talked about them briefly in episode 18, and maybe it touched on them otherwise, but

0:58.1

we're going to dive into them in a bit more depth in this episode.

1:01.1

First, I'm going to just talk a little bit about some of the methods that we use to study

1:04.4

proteins, and then I'm going to talk about protein structure and protein functions.

1:09.1

So that's just how proteins are structured, what they look like, and how they fit together, and protein functions. So that's just how proteins are structured, what they

1:12.4

look like and how they fit together. And protein function is how they behave and what they do,

1:16.7

with a focus on looking at proteins as enzymes and protein foley. So let's jump straight in.

1:23.4

First, as I said, we'll talk a bit about the methods used to study proteins, because some of them are

1:27.1

quite interesting. So when we want to study proteins, the I said, we'll talk a bit about the methods used to study proteins, because some of them are quite interesting.

1:28.3

So, when we want to study proteins, the first step that we need to do is obtain a pure sample of the protein.

1:35.3

So just to remind you, proteins are a type of macromolecule found in all living organisms.

1:42.3

They consist of a sequence of amino acids strung together,

1:45.6

usually hundreds or thousands of amino acids, and they fold up in a particular three-dimensional

1:50.8

structure, which determines their function. And proteins are responsible for a very wide variety

1:56.1

of functions in the cell, including acting as messengers and as transportation molecules and as signaling molecules and structural molecules, all sorts of things.

...

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