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MCAT Basics (from MedSchoolCoach)

The Krebs Cycle

MCAT Basics (from MedSchoolCoach)

MedSchoolCoach

Science

4.6612 Ratings

🗓️ 26 June 2025

⏱️ 32 minutes

🧾️ Download transcript

Summary

One of the most fundamental biochemical processes is the Krebs cycle. This metabolic pathway plays a critical role in both the Chem Phys and Bio/Biochem sections of the MCAT, so understanding it is key.

In this episode, our guest host, Alex Starks, walks us through the transformation of pyruvate into acetyl CoA via the Pyruvate Dehydrogenase Complex (PDC). We’ll explore how thioester bonds help transfer energy within the cycle, how acetyl CoA combines with oxaloacetate to form citrate, the difference between enzymes like synthetases and synthases, and how GTP is produced. We’ll also make connections to the electron transport chain and discuss how the TCA cycle influences blood pH through CO2 production.

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Jump into the conversation:

(00:00) Intro

(01:05) Recap of glycolysis and pyruvate

(02:45) Pyruvate dehydrogenase complex (PDC)

(03:40) Role of acetyl CoA in the Krebs cycle

(05:37) How citrate is formed 

(07:17) How isocitrate is formed

(10:00) How alpha-ketoglutarate is formed

(13:42) How  succinate and GTP are formed

(16:28) How succinate, fumarate and oxaloacetate are formed

(18:23) Fumarate converted to malate

(21:53) Recap of the Krebs cycle and ATP yield

(25:00) Regulation of the Krebs cycle

(26:16) Quiz

 

Transcript

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0:00.0

Welcome to MCAT Basics, your ultimate guide to the essential topics you need to master for the MCAT,

0:06.2

brought to you by the physicians at med school coach. Every week, Sam Smith breaks down high-yield

0:11.5

MCAT topics, ensuring you're primed for success on test day. Join Sam as we explore the most

0:17.2

crucial subjects outlined by the AAMC, pulled from official practice materials and third-party resources.

0:23.3

Get ready to elevate your MCAT game with topics tailored to maximize your score potential.

0:28.6

Today's guest host is Alex Starks.

0:31.3

Hello, our goal for today is to master more metabolism, specifically the CRIF cycle, also known as the citric acid cycle,

0:38.5

and also known as the TCA or tricarboxylic acid cycle. So this content is really important

0:43.7

for one major reason. It shows up in both chem-physsection, as well as the bio-biocan

0:50.5

section. This episode is also a continuation in our series on metabolism, and if you recall,

0:56.2

on our previous metabolism podcast, we discussed glycolysis. We focused on that metabolic process as,

1:02.0

like, quote, the robbing of electrons from glucose in order to power an insatiable power factory

1:07.7

called the mitochondria. Where we left off was focusing on the final product of

1:12.2

glycolysis, this three carbon molecule called pyruvate. And I want to stress, again, like the

1:18.7

structure of this molecule being very important. So I'll just review it real quick. So carbon one is a

1:24.4

carboxylate, so a C-O-0 minus. Carbon two is a ketone, so C-double bondo, and then carbon 1 is a carboxylate, so a C-O-0-9. Carbon 2 is a ketone, so C double bondo.

1:30.3

And then carbon 3 is just a boring carbon has three hydrants connected to it.

1:35.3

So last episode, we also discussed how pyruid's journey can continue either in the presence

1:39.3

or absence of oxygen.

1:41.3

So today we're going to continue following the oxygen present or aerobic fate of

1:46.6

pyruvate.

1:53.6

So the first thing that happens is that it's going to enter into the mitochondria because glycolysis

...

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