LIVE: FBI Agent Breaks Down What's Left of the Guthrie Evidence
True Crime Today | Daily True Crime News & Interviews
Tony Brueski
4.2 • 612 Ratings
🗓️ 23 February 2026
⏱️ 15 minutes
🧾️ Download transcript
Summary
Jennifer Coffindaffer joins Hidden Killers Live to assess the physical evidence in the Nancy Guthrie case — and separate what's real from what's noise. The glove found two miles from the house has dominated headlines, but its DNA doesn't match anything at the property and hit nothing in CODIS. Coffindaffer tackles the question no one else is asking: should this glove even be treated as evidence in this case?
Inside the home, the DNA picture is a mess — a mixture of multiple contributors that investigators are still trying to separate. Coffindaffer explains what that process actually involves, what determines success or failure, and whether the sample is usable for the genetic genealogy approach investigators are now pursuing.
The lab fight gets a direct assessment: Nanos sent evidence to a private Florida lab while the FBI wanted it at Quantico. Coffindaffer evaluates both arguments and addresses whether evidence may have already been degraded. The conversation also covers Google's admission that additional Nest footage likely cannot be recovered, the ongoing pacemaker search, and why 50,000 tips haven't produced a suspect.
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Transcript
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| 0:00.0 | This is Hidden Killers with Tony Bruske. |
| 0:03.0 | Here now, Tony Brewski. |
| 0:06.1 | Here we are. |
| 0:08.4 | Three weeks in, entering our fourth week into the disappearance of Nancy Guthrie. |
| 0:15.7 | And still, no answers, at least as of our recording right here DNA found inside the home mixture it is still |
| 0:25.3 | being separated to be tested there's a glove that was found two miles away it did not have any |
| 0:31.6 | sort of codis match but it had DNA on it allegedly genetic genealogy is now in play here on at least that one, and I'm |
| 0:40.1 | sure maybe the other will find out. Google says they probably can't pull more camera footage, |
| 0:47.0 | and the lab that's processing everything isn't the, well, it initially was not the FBI's. |
| 0:53.0 | Jennifer Coffin-Daffer, retired FBI special agent, is with us to help break down where we're at in this nightmare of a story that just doesn't seem to end. |
| 1:03.6 | Jen, let's talk about the DNA profiles, because now we essentially what we understand is we have two. |
| 1:09.5 | There's some sort of a DNA profile in the house. |
| 1:11.8 | That's the mixed one. |
| 1:13.0 | There's the one on this glove that was found two miles away. |
| 1:16.1 | And now they're saying that they're going to go to genetic genealogy to get more on that. |
| 1:22.6 | I want to talk both of them, but they're both very different. |
| 1:26.1 | Let's talk about the glove really quick. |
| 1:29.3 | Should we have any sort of weight on this glove whatsoever, I guess, is my question. |
| 1:34.6 | It's such a great question. And from the beginning, we've known, first of all, the proximity, |
| 1:39.9 | you know, over two miles away and the fact that it was in a field of gloves, right? |
| 1:45.8 | 16 in various locations far away from the house. |
| 1:49.3 | But they identified this one, I think, is matching, you know, the consistency of the gloved hand |
... |
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