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Science Quickly

Microbiome Studies Contaminated by Sequencing Supplies

Science Quickly

Scientific American

Science

4.2639 Ratings

🗓️ 11 November 2014

⏱️ 2 minutes

🧾️ Download transcript

Summary

Nonsterile lab reagents and DNA extraction kits add their own assortment of DNA to microbiome samples. Christopher Intagliata reports Learn more about your ad choices. Visit megaphone.fm/adchoices

Transcript

Click on a timestamp to play from that location

0:00.0

Understanding the human body is a team effort. That's where the Yachtel group comes in.

0:05.8

Researchers at Yachtolt have been delving into the secrets of probiotics for 90 years.

0:11.0

Yacold also partners with nature portfolio to advance gut microbiome science through the global grants for gut health, an investigator-led research program.

0:19.6

To learn more about Yachtolt, visit yawcot.co.j.j, that's y-A-K-U-L-T-C-O-J-P.

0:28.4

When it comes to a guide for your gut, count on Yacolt.

0:33.4

This is Scientific American 60-second science.

0:36.6

I'm Christopher indalata.

0:37.9

Got a minute?

0:39.4

In this age of cheap DNA technology, scientists are sequencing every sample they can get their hands on.

0:45.6

They've IDed the microbes in mosquito guts, coral mucus, and frog skin, in polar ice, even floating in the Earth's atmosphere.

0:58.0

But it turns out some of the bugs reported to belong to those unusual microbiomes could unfortunately be contaminants from non-sterell lab reagents and DNA extraction kits.

1:05.0

So says a study in the journal BMC Biology.

1:08.0

Researchers sequenced a pure sample of just one type of bacteria, but depending

1:13.1

which kit they used, which reagents, which lab, their results contain DNA from up to 270 different

1:19.8

bacterial strains. Many of those contaminating strains are commonly found on human skin, a lab

1:26.1

technicians maybe, or in soil or water, which could

1:29.9

explain why one recent study turned up soil bacteria in samples of breast cancer tissue, the

1:35.7

researchers say. Another study found that infant's throat bacteria change as they get older,

1:41.0

but these researchers say the changing bacterial communities in that study

1:44.9

were due not to age, but to changing the brand of DNA kit over time.

1:50.3

Study author Alan Walker of the University of Aberdeen says contamination is only a problem if you're

1:55.6

working with samples that aren't already rich in bacteria.

...

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